Reversible Gels for Electrophoresis and Isolation of DNA
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چکیده
منابع مشابه
Reversible gels for electrophoresis and isolation of DNA.
Here, the application of the gel-forming carbohydrate polymer, gellan gum, for the electrophoresis and isolation of DNA is detailed. Gellan gun forms gels in the presence of divalent metal cations, and the gels can be converted back to a solution by the addition of a chelating agent such as EDTA. Also, gellan electrophoresis gels can be formed using diamines. These gels are reversible by increa...
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A problem that arises when cloning DNA is the isolation of specific DNA molecules following restriction enzyme digestion or PCR amplification. Typically, a mixture of DNA fragments is electrophoretically separated by agarose gel electrophoresis, the gel is stained with ethidium bromide, and the DNA is visualized by UV transillumination. The DNA fragments of interest are excised from the gel usi...
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Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99264rr04